ykuI [2019-09-23 10:21:57]

c-di-GMP binding protein

locus: BSU_14090

pI: 4.46

mw: 47.80 kDa

protein length: 407 aa

gene length: 1224 bp

function: unknown

product: c-di-GMP binding protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.2|Targets of c-di-GMP]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,482,248 1,483,471

Phenotypes of a mutant

• inactivation of ''[gene|A9C45C522EAF534C41CB75AA364A379E1D11055F|ykuI]'' confers resistance to high concentrations of Zn(II) [Pubmed|27935957]

The protein

[SW|Domains]

• EAL domain (aa 1-250) (according to UniPort), PAS-like domain (aa 300-400) [Pubmed|19244251]

Structure

• [PDB|2BAS]
• in complex with c-di-GMP [PDB|2W27] [Pubmed|19244251]

Expression and Regulation

Operons

• genes: [gene|69E475F00D9CCFDDF086F0B8A5CBA81B31DD06FE|ykuH]-[gene|A9C45C522EAF534C41CB75AA364A379E1D11055F|ykuI]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A770 (ykuI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/770 NBRP B. subtilis, Japan]
• GP1324 (tet), available in [SW|Jörg Stülke]'s lab
• BKE14090 ([gene|A9C45C522EAF534C41CB75AA364A379E1D11055F|ykuI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCTGCTTCTC, downstream forward: _UP4_TAACGGCTGAAAGGCCGTTT
• BKK14090 ([gene|A9C45C522EAF534C41CB75AA364A379E1D11055F|ykuI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCTGCTTCTC, downstream forward: _UP4_TAACGGCTGAAAGGCCGTTT

References

• 23893111,19244251,22821967,27116468,27935957